Biotin & Streptavidin Conjugation Services

Biotin-streptavidin conjugation is a powerful and widely used method in biotechnology for forming strong, non-covalent bonds between biotin (a small molecule also known as vitamin B7) and streptavidin (a protein with high affinity for biotin). This conjugation technique is commonly used in molecular biology for applications such as immunoassays, cell targeting, and biomolecule purification, due to its unparalleled binding strength.

Biotin-streptavidin conjugation offers unmatched specificity and binding strength, which makes it ideal for applications requiring a stable and robust linkage. This method minimises background noise in assays and increases the reliability of experimental results, making it superior for sensitive biological applications.

In diagnostics and research, biotin-streptavidin conjugation is used for enhancing signal detection in ELISA tests, facilitating efficient biomolecule separation in affinity chromatography, and improving the accuracy of molecular imaging techniques. Its ability to strongly and specifically bind components makes it essential for developing precise diagnostic tools.

A wide range of biomolecules can be conjugated using biotin and streptavidin, including antibodies, peptides, nucleic acids, and even small molecules. The versatility of this conjugation method allows it to be adapted for various molecular weights and sizes, providing flexibility in biochemical and clinical research.

While biotin-streptavidin conjugation is highly effective, it may not be suitable for all applications due to the high binding affinity, which can make dissociation difficult without denaturing conditions. Additionally, the cost of pure streptavidin can be a consideration for large-scale applications.

When handling biotin-streptavidin conjugates, it is important to avoid conditions that might disrupt the biotin-streptavidin interaction, such as extreme pH or high temperatures. Proper storage and handling are crucial to maintain the stability and functionality of these conjugates.

We customise biotin-streptavidin conjugation services based on the specific needs of our clients by selecting appropriate conjugation ratios, optimising the conjugation process for specific biomolecules, and tailoring the purification processes to ensure the highest activity and purity levels necessary for the intended application.