Antibody Digestion Services
Antibody digestion is a specialised technique in the field of biochemistry and immunology that involves the cleavage of antibodies into smaller, functional fragments.
The primary purpose of antibody digestion is to generate fragments such as Fab, Fab' and F(ab')2, which can be used in various applications. These fragments all retain antigen-binding capabilities but lack the Fc region, making them ideal for reducing non-specific binding in immunodiagnostic procedures.
Antibody Digestion
Antibody digestion is vital for both diagnostic and therapeutic uses. Employing a range of enzymatic methods, we can precisely cleave antibodies into functional fragments.
Previous Projects
IgM Digestion
The project focused on the digestion of IgM antibodies for an in vitro diagnostic (IVD) company. This initiative involved developing a precise and efficient enzymatic digestion process, which was aimed at preparing fragments for use in various diagnostic assays and applications.
Fab' Generation & Conjugation to Biotin
The project involved the preparation of Fab' fragments from full monoclonal antibodies and their subsequent conjugation to biotin. This process was designed to enhance the specificity and utility of the Fab' fragments in biotin-based detection systems, supporting their use in diagnostic and research applications.
F(ab')2 Generation and Conjugation to Fluorescent Dye
The project focused on the preparation of F(ab')2 fragments from monoclonal antibodies and their subsequent conjugation to fluorescent dyes. This initiative was designed to enhance the detection capabilities of these antibody fragments in various bioimaging and diagnostic applications.